Zinc-coordination and C-peptide complexation: A potential mechanism for the endogenous inhibition of IAPP aggregation

X Ge; A Kakinen; EN Gurzov; W Yang; L Pang; EH Pilkington; P Govindan-Nedumpully; P Chen; F Separovic; TP Davis; PC Ke; F Ding

Journal article

Zinc-coordination and C-peptide complexation: A potential mechanism for the endogenous inhibition of IAPP aggregation

X Ge, A Kakinen, EN Gurzov, W Yang, L Pang, EH Pilkington, P Govindan-Nedumpully, P Chen, F Separovic, TP Davis, PC Ke, F Ding

Chemical Communications | ROYAL SOC CHEMISTRY | Published : 2017

DOI: 10.1039/c7cc04291d

Abstract

Aggregation of the highly amyloidogenic IAPP is endogenously inhibited inside beta-cell granules at millimolar concentrations. Combining in vitro experiments and computer simulations, we demonstrated that the stabilization of IAPP upon the formation of zinc-coordinated ion molecular complex with C-peptide might be important for the endogenous inhibition of IAPP aggregation.

University of Melbourne Researchers

Frances Separovic Author

Related Projects (1)

ARC CENTRE OF EXCELLENCE IN CONVERGENT BIO–NANO SCIENCE AND TECHNOLOGY

The CoE in Convergent Bio-Nano Science &Technology comprises a multi-disciplinary team focused on research aiming to understand and control ..

Grants

Awarded by National Institute of General Medical Sciences

Funding Acknowledgements

The work is partially supported by ARC Project No. CE140100036 (Davis), NSF CAREER CBET-1553945 (Ding), NIH R35GM119691 (Ding), NHMRC Project Grant APP1071350 (Gurzov), and an internal grant from Monash Institute of Pharmaceutical Sciences (Ke). Gurzov is supported by a Juvenile Diabetes Research Foundation (JDRF) fellowship. The content is solely the responsibility of the authors and does not necessarily represent the official views of NIH and NSF.